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Showing: 6201 - 6220 of 14407 results

  2. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172835-plasmid-map-sequence-id-341082

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  3. pKT3Ts-goldyTALEN

    Plasmid
    #80330
    Purpose
    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence
    Depositor
    Jeffrey Essner
    Article
    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi
    Type
    Empty backbone
    Use
    TALEN
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T3
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80330-plasmid-map-sequence-id-156328

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  7. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

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  20. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52907
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Mutation
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6201 - 6220 of 14407 results