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Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLenti-TagGFP2
Plasmid#121426PurposeTAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertempty TagGFP2
UseLentiviralTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbV5
Plasmid#201476PurposeNanoBit detection of V5-tagged proteinDepositorInsertLgBit-NbV5
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4 DD
Plasmid#26999DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationC867APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCX-mutSpiCee
Plasmid#140903Purposemammalian expression of a mutated form of SpiCee, that does not affect calcium signalingDepositorInsertmutSpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls
Plasmid#64112PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_HP1
Plasmid#82607PurposeExpresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cellsDepositorInsertsfCherry2(11)_HP1b
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-LOV*-MVP
Plasmid#202206PurposeExpresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cellsDepositorInsertMajor vault protein (MVP Human)
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt9A
Plasmid#35918DepositorInsertWnt9A (WNT9A Human)
UseTagsExpressionMammalianMutationSilent Mutation A (543) --> GPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 CFP
Plasmid#19755DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsCFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-Limk1
Plasmid#163414PurposeExpresses LIMK1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEmCherry2
Plasmid#118413PurposeRed fluorescent reporter for assessing CRISPR gRNA activityDepositorInsertmCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only