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Plasmid#191825PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-GW
Plasmid#61702PurposeGateway compatible Y2H vector - ADDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
piRFP-N3-Tyrosinase
Plasmid#80152PurposeMelanosome markerDepositorInsertTyrosinase (TYR Human)
UseTagsiRFP682ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterEF1 alpha and TREAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD3 delta YFP
Plasmid#11951DepositorInsertCD3delta-YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-BsaIx2-DD-Cas9
Plasmid#75380PurposeExpresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1DepositorInsertDD-SpCas9
UseCRISPRTagsFKBP12 L106P DDExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 bovine GRK2
Plasmid#14691DepositorInsertGRK2 (GRK2 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
h-alpha-ENaC
Plasmid#83430Purposefull length human alpha ENaC with Flag tagDepositorInserthuman alpha ENaC (SCNN1A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-miR21
Plasmid#20381DepositorInsertmiR21 (MIR21 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato
Plasmid#44332DepositorHas ServiceAAV5InserttdTomato
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-STING-sh5
Plasmid#127647PurposeKnock-down of human STINGDepositorInsertSTING shRNA (STING1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-97)PromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only