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Plasmid#113937PurposeBacterial expression of red fluorescent Ca2+ sensor R-GECO1.2DepositorInsertR-GECO1.2
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-TNFalpha
Plasmid#20210DepositorInsertTNFa 3' UTR (TNF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
UseTagsMGHHHHHHGGAExpressionMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW2341
Plasmid#186950Purpose30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linkerDepositorInsert30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FKBP-GFP-HIS3MX6
Plasmid#183023PurposePCR-based tagging of yeastDepositorInsertFKBP
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 Q101L
Plasmid#86788Purposemammalian expression of Rab12 Q101L, constitutively GTP-bound mutantDepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationQ101L, constitutively GTP-bound mutantPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
yWL234 MCP-GFP-PABPC1*
Plasmid#197054PurposepUBC-NLS-HA-2xMCP-1xGFP-PABPC1*DepositorInsertpubc-nls-ha-MCP-GFP-PABPC1*
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptet1
Plasmid#218367PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-L26-Flag
Plasmid#19972DepositorInsertRpl26 (RPL26 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-ER-ZapCY1
Plasmid#36321DepositorInsertER-ZapCY1 high affinity Zn(II) sensor
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC118-FLIP-Puro
Plasmid#84538Purposea mock vector for generating FLIP targeting vectorsDepositorInsertFLIP - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTmpSm
Plasmid#176228PurposeA template plasmid for amplification of the cI-hok-strA cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, strA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL1
Plasmid#182263PurposeGibson cloning vector for synthesis of single and double stranded RNADepositorTypeEmpty backboneUseGibson cloning vector for synthesis of single and…TagsExpressionMutationPromoterT3 and SP6 for sense and antisense riboprobe synt…Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ZapCY2
Plasmid#36320DepositorInsertZapCY2 high affinity Zn(II) sensor
UseTagsExpressionMammalianMutationsee commentPromoterCMVAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCd1-mNGx1
Plasmid#202751Purposeexpresses fluorescent protein-tagged lipid biosensorDepositorInsertPLCD1v2(1-170):mNG2 (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.G
Plasmid#200999PurposepLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT13.G
Plasmid#201001PurposepLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only