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mEGFP-N1 YAPS127A- L325E

Plasmid

#166464

Purpose

Expresses fusion of mEGFP and YAPS127A- L325E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L325E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L325E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

Plasmid

#172635

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ser

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup-deltaC

Plasmid

#90023

Purpose

To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Deletion of C-terminal region

Promoter

CMV

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-CAGGS-ERAS

Plasmid

#52416

Purpose

Lentiviral vector encoding constitutive expression of human ERAS cDNA

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

Plasmid

#170196

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pNTK human MBD3 PGK-Neo-pA flox

Plasmid

#52362

Purpose

Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

MBD3 (MBD3 Human)

Use

Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-WT-neo

Plasmid

#156177

Purpose

Lentiviral vector for expression of RAP2A-WT, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-N17-neo

Plasmid

#156179

Purpose

Lentiviral vector for expression of RAP2A-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

Plasmid

#172625

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr280Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1-Puro

Plasmid

#172643

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 M C-V5-tag

Plasmid

#179986

Purpose

Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 M

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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W118-1_hRSK3(RPS6KA2)_K91A_K444A

Plasmid

#180385

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2_K91A/K444A (RPS6KA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K91A/K444A

Promoter

CMV

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

Plasmid

#170188

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

Promoter

CMV51

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L318E

Plasmid

#166465

Purpose

Expresses fusion of mEGFP and YAPS127A- L318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L318E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L318E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge