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Plasmid#78672PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_EB
Plasmid#78671PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_AB
Plasmid#78670PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOGGFPR
Plasmid#64398PurposeOat globulin Glo1 promoter driving expression of GFPDepositorInsertOat globulin Glo1 promoter
UseTagsGFPExpressionPlantMutationPromoterOat globulin Glo1Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL11c
Plasmid#17833DepositorInsertalcA promoter, lacZ, argB_1626,
UseSynthetic Biology; Aspergillus nidulans expressionTagsExpressionMutationThe argB_1626 mutation consists of two sequential…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…ExpressionMutationPromoterSOX10 Multiple Species Conserved enhancer element…Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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EF1α-mNeonGreen-Z1
Plasmid#135613PurposemNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.DepositorInsertmNeonGreen
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1α-eGFP-Z1
Plasmid#135612PurposeEnhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPARA2
Plasmid#200417PurposeGolden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53206PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and TRP1ExpressionMutationPromoterGAL1 promoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
p404GALS
Plasmid#17435DepositorInsertGALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALS promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6ExpressionMutationPromoterGal1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53207PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and HIS3MX6ExpressionMutationPromoterGAL1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only