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Plasmid#199364PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42
Plasmid#153525PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#42DepositorInsertSb#42
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorInsertSb#15
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP
Plasmid#199368PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#14
Plasmid#153522PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#14DepositorInsertSb#14
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-GBP
Plasmid#67672PurposeMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)DepositorInsertanti-GFP nanobody
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-SHMY-PAUF
Plasmid#182645PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequenceDepositorInsertPancreatic adenocarcinoma upregulated factor (PAUF)
UseRetroviralTagsHis6-tag, N-terminal ER targeting sequence of hem…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenusC-Cavin3
Plasmid#108890PurposeExpression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertCavin3 (altered codon usage) (CAVIN3 Synthetic, Human)
UseTagsmVenus156-239ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceMarch 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-Lag16-fibcon-iCAM1-tether
Plasmid#205213PurposeThis vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
UseTagsmodified STIM1-PB tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbSARS
Plasmid#171703PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_NbH11-D4
Plasmid#155363PurposeExpresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertNb H11-D4
UseTagsHis6ExpressionMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_H3
Plasmid#171927PurposeBacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_H3
UseTagsHis6ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_F2
Plasmid#171926PurposeBacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_F2
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
HMA-APOBEC1-Nb
Plasmid#226012PurposeFor expression of His-MBP-APOBEC1-Nanobody protein in E.coliDepositorInsertAPOBEC1 (Apobec1 Rat)
UseTagsHis-MBP and antiRabbit-nanobodyExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -