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Plasmid#179446PurposeRetroviral expression vector containing the DivisionRecorder cassetteDepositorInsertGFP-P2A-511-CrePI-511
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-nsp7-FLAG
Plasmid#201027PurposeExpresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTags8xHis and FLAGExpressionMammalianMutationPromoterT7 promoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-repo1.9kb-TALE4-VP64-P10
Plasmid#104612PurposeExpresses TALE4 under the control of a 1.9kb repo enhancer elementDepositorInsert1.9kb repo enhancer element (repo Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-hFADD-FLAG
Plasmid#31814DepositorInsertFADD (FADD Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-K274W
Plasmid#55690Purposeexpresses dominant negative human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120E and K274WPromoterCMVAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-A120E
Plasmid#55687Purposeexpresses constitutively active human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120EPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)
Plasmid#183254PurposeExpression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vectorDepositorInsertTissue factor (F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.sgKras.9
Plasmid#91894PurposesgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.DepositorInsertKras sgRNA
UseLentiviralTagsExpressionMutationPromoterU6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pJC-ase0.8kb-TALE3-VP64-P10
Plasmid#104610PurposeExpresses TALE3 under the control of a 0.8kb ase enhancer elementDepositorInsert0.8kb ase enhancer element (ase Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His
Plasmid#195610PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tagDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT825C, C900G, A901C, A903C, T1131G, T1137C, T1227…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 R159K
Plasmid#123626PurposeExpresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationArg159LysPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2 H12A
Plasmid#123619PurposeExpresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationHis12AlaPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only