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mCGN-Myc-His

Plasmid

#236510

Purpose

For expression of mCingulin in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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hCGNL1-Myc-HA

Plasmid

#236515

Purpose

For expression of hParacingulinL1 in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (CGNL1 Human)

Use

Tags

Myc-HA

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-Myc

Plasmid

#236509

Purpose

For expression of mCingulin in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ASAP3(Cterm)

Plasmid

#221304

Purpose

Expression of ASAP3 (Cterm pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ASAP3 (Cterm pLxIS) (Asap3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1021

Plasmid

#143906

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZXDC (ZXDC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1020

Plasmid

#143905

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZXDC (ZXDC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3469

Plasmid

#144945

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF438 (ZNF438 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2476

Plasmid

#144030

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFHX2 (ZFHX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_297-370

Plasmid

#196529

Purpose

Entry vector for Gateway with Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2

Plasmid

#196542

Purpose

Expression of myc-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Use

Tags

mWasabi fluorescent tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001149537)

Plasmid

#77927

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001487065)

Plasmid

#77928

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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POMK gRNA (BRDN0001146370)

Plasmid

#77758

Purpose

3rd generation lentiviral gRNA plasmid targeting human POMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

POMK (POMK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POMK gRNA (BRDN0001147737)

Plasmid

#77759

Purpose

3rd generation lentiviral gRNA plasmid targeting human POMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

POMK (POMK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POMK gRNA (BRDN0001146669)

Plasmid

#77760

Purpose

3rd generation lentiviral gRNA plasmid targeting human POMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

POMK (POMK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
POMK gRNA (BRDN0001144851)

Plasmid

#77761

Purpose

3rd generation lentiviral gRNA plasmid targeting human POMK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

POMK (POMK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SRPK3 gRNA (BRDN0001146140)

Plasmid

#77396

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK3 (SRPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge