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Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.3t
Plasmid#210255PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsDsRed
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
OA984-HA
Plasmid#120362PurposeExpresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporterDepositorInsert1C19 his-tagged single chain antibody
UseUnspecifiedTagsExpressionMutationPromoterAAEL010782 carboxypeptidase A (CPA) gene PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-humanTRIM5-FOS
Plasmid#79066PurposeExpress human TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(human)-FOS (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSAR-Cre-F2A-I-SceI
Plasmid#62302PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed markerDepositorInsertCre recombinase, I-SceI nuclease
UseTagsExpressionInsectMutationPromotervasaAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS
Plasmid#79067PurposeExpress owl monkey TRIMCyp in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2
Plasmid#161540PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoterDepositorInsert3xFLAG-NLS-DsRed-ZF1-PEST
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF10x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDS-RED-C1-Beclin1
Plasmid#24405DepositorInsertBeclin1 (BECN1 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet
Plasmid#104006PurposePlasmid encoding mScarletDepositorInsertmScarlet
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEF… V…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-whiteCopyCatcher
Plasmid#174062PurposeCopyCatcher insertion donor for the white locusDepositorInsertwhite (w Synthetic, Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_tdTomato
Plasmid#135178PurposeAAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent lablingDepositorInsertstdTomato
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of DsRedPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-AGMpygTRIM5-FOS
Plasmid#79064PurposeExpress African green monkey pygerythrus TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(African green monkey pygerythrus)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIV-dual-GT
Plasmid#122696PurposeDual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.DepositorInsertsmTagBFP2
DsRed-max
UseLentiviralTagsPEST domainExpressionMutationPromoterHIV-1 LTRAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gr4-T2A-QF2 HDR plasmid
Plasmid#140944PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 geneDepositorInsertGr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
UseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only