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Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140229PurposeDox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFPDepositorInsertAntiCRISPR protein AcrVA1
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5079_pHR_PGK_sfGFP_CoV-F1
Plasmid#155303PurposeSARS-CoV-2 fluorescent reporter 1DepositorInsertSuperfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_Ntag_BbsI-Mammalian
Plasmid#186674PurposeN-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.DepositorInsertMammalian N-terminal Tag
UseTags3xFlag-3xHAExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2
Plasmid#190898PurposeEntry vector for multiple sgRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6 and 7skAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-S45
Plasmid#164587PurposeExpresses a gRNA that overlaps the S45 codon of human CTNNB1DepositorInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mScI-CTNNB1
Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-CTNNB1-ATG
Plasmid#153429PurposeExpresses a gRNA that overlaps the startcodon of human CTNNB1DepositorInsertCTNNB1 gRNA (CTNNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SYFP2-CTNNB1
Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF738
Plasmid#122032PurposeEF1a-WNV-protease-GFPDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5080_pHR_PGK_sfGFP_CoV-F2
Plasmid#155304PurposeSARS-CoV-2 fluorescent reporter 2DepositorUseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-GFP(1-10)opti
Plasmid#129416PurposeExpressing codon-optimized GFP(1-10) fragment in human cellsDepositorInsertcodon-optimized GFP(1-10)
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor
Plasmid#211888PurposeCENP-T[1-242] transgene integration at AAVS1 safe harbor locusDepositorInsertCENP-T[1-242] (CENPT Human, Synthetic)
UseCRISPRTagsExpressionMutationT11A, T85APromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only