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Showing: 1501 - 1550 of 1663 results

  1. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  3. pIK198

    Plasmid
    #65629
    Purpose
    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65629-plasmid-map-sequence-id-111540

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  6. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  17. pET11a-Z-N-DiB2

    Plasmid
    #168473
    Purpose
    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2 + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168473-plasmid-map-sequence-id-332731

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  18. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

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  19. pMRBad-Z-C-wtBlc

    Plasmid
    #168256
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    Leucine Zipper + C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168256-plasmid-map-sequence-id-332726

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  20. JPF0521

    Plasmid
    #124012
    Purpose
    Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pPGK1c-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124012-plasmid-map-sequence-id-252423

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  21. JPF0523

    Plasmid
    #124014
    Purpose
    Encodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pSFFVc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124014-plasmid-map-sequence-id-252431

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  26. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  28. pHES830

    Plasmid
    #87944
    Purpose
    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    87944-plasmid-map-sequence-id-180365

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  29. pGCNK58

    Plasmid
    #202461
    Purpose
    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
    Depositor
    Arthur Palmer, Stephen Harrison
    Article
    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi
    Insert
    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202461-plasmid-map-sequence-id-403118

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  39. pR I-Ab alpha BirA

    Plasmid
    #113638
    Purpose
    Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site
    Depositor
    James Moon
    Article
    Moon et al Methods Mol Biol. 2018;1799:165-181. doi
    Insert
    I-Ab alpha BirA (H2-Ab1 Mouse)
    Use
    Tags
    BirA biotinylation signal site and fused to Fos-J…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113638-plasmid-map-sequence-id-221155

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Showing: 1501 - 1550 of 1663 results