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pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Plasmid

#221776

Purpose

Expresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191174

Purpose

Mammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJB004 CMV 3xFLAG-NLS-VP16-intN

Plasmid

#161549

Purpose

Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP16-intN

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJB005 CMV 3xFLAG-NLS-intC-ZF2

Plasmid

#161550

Purpose

Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-intC-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

Plasmid

#185913

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

Plasmid

#185918

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HAT1_AG

Plasmid

#217113

Purpose

Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT1_AG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TC1_FR4

Plasmid

#217148

Purpose

Mammalian expression vector for expression of TC1_FR4 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TC1_FR4 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TC1_DM

Plasmid

#217154

Purpose

Mammalian expression vector for expression of TC1_DM transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TC1_DM transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TC1_FR2

Plasmid

#217156

Purpose

Mammalian expression vector for expression of TC1_FR2 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TC1_FR2 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-S2_DM

Plasmid

#217161

Purpose

Mammalian expression vector for expression of S2_DM transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

S2_DM transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PROTOP

Plasmid

#217162

Purpose

Mammalian expression vector for expression of PROTOP transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

PROTOP transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Mariner2_AG

Plasmid

#217135

Purpose

Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Mariner2_AG transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1DR3_Xt

Plasmid

#217139

Purpose

Mammalian expression vector for expression of Tc1DR3_Xt transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1DR3_Xt transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TC1_XL

Plasmid

#217144

Purpose

Mammalian expression vector for expression of TC1_XL transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TC1_XL transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myotis_hAT1

Plasmid

#217146

Purpose

Mammalian expression vector for expression of Myotis_hAT1 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Myotis_hAT1 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HAT2_CI

Plasmid

#217107

Purpose

Mammalian expression vector for expression of HAT2_CI transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT2_CI transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HAT5_CI

Plasmid

#217108

Purpose

Mammalian expression vector for expression of HAT5_CI transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

HAT5_CI transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Chaplin1_DR

Plasmid

#217110

Purpose

Mammalian expression vector for expression of Chaplin1_DR transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Chaplin1_DR transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-POGO

Plasmid

#217116

Purpose

Mammalian expression vector for expression of POGO transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

POGO transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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