-
Plasmid#90307Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationD422APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
UseTags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-dWPRE
Plasmid#60907PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 BRE Luciferase
Plasmid#45126DepositorInsertId1-BRE
UseLuciferase; Bmp/smad transcriptional reporterTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-UBE2E1 WT in pcDNA3.1
Plasmid#118213PurposeExpresses HA-UBE2E1 in mammalian cellsDepositorInsertUBE2E1 (UBE2E1 Human)
UseTagsHAExpressionMammalianMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Tax WT
Plasmid#23284DepositorInsertTax
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-HA-UbcH13
Plasmid#12461DepositorInsertUbc13 (UBE2N Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRF
Plasmid#66936PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV-40Available sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gq sensor
Plasmid#137810PurposeA FRET sensor for Gq activation, encoded on a single plasmidDepositorInsertGq sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp2
Plasmid#192782Purposegreen biosensor for cAMP in mammalian cellsDepositorInsertG-Flamp2
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO-cell-tagRFP
Plasmid#185598PurposeLentiviral construct for expression of tagRFP-TDepositorInsertcell-tagRFP-T
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV Rac
Plasmid#50535DepositorInsertsmall GTPase (RAC1 Human)
UseTags2X VSVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pink Flamindo
Plasmid#102356Purposebiosensor for cAMP in mammalian cellsDepositorInsertPink Flamindo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterNAAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry
Plasmid#207668PurposeTo express fluorescently labeled MS2 coat binding protein (MCP)DepositorInsertMCP-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEP179_pX330K
Plasmid#137882PurposeEntry vector for multi-gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only