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Plasmid#33328DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationDeleted RP domain (amino acids 167-189 missing)PromoterSV40Available sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Dom34(1-138)
Plasmid#19101DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 2818 (=HL 865 + pHL 489)
Plasmid#30032DepositorInsertpLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-deltaNLS1
Plasmid#22153DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationdeletion of amino acids 297-304PromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHL 1011
Plasmid#52993PurposepLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfpDepositorInsertsRhyB sRNA
RBS (st7)
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationPromoterpLlac0-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR
Plasmid#61779PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1DepositorInsertmouse Xbp1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pP2A11 (GB0082)
Plasmid#68168PurposeProvides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid partDepositorInsertSl2A11 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat 1-460 K193A
Plasmid#33326DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationtruncated at 460, K193A (S3V, G96R, and P108L als…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat 1-460 D311A
Plasmid#33325DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationtruncated at 460, D311A (S3V, G96R, and P108L als…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p7012 MSCV-IP N-HAonly 18E6nonsplice
Plasmid#44148DepositorInsertHPV18E6
UseRetroviralTagsHAExpressionMammalianMutationInternal splice site mutatedPromoterMSCV LTRAvailable sinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
HL 4218 (=HL 716 + pHL 1411)
Plasmid#30105DepositorInsertpLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsT7noRBS, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4212 (=HL 716 + pHL 1414)
Plasmid#30104DepositorInsertpCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsT7noRBS, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
GUCY1B3
Plasmid#39121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationThis is a complex of two proteins; GUCY1A3 (aa 46…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS1044
Plasmid#63797PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection markerDepositorInsert3xFLAG followed by KlLEU2
UseE. coli cloning vectorTags3xFLAGExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Paxillin-22
Plasmid#57849PurposeLocalization: Focal Contacts, Excitation: 548, Emission: 561DepositorInsertPaxillin
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCplac111-KOG1 (pAN58)
Plasmid#39209DepositorInsertKOG1 (KOG1 Budding Yeast)
UseTagsmycExpressionYeastMutationL1512PPromoterKOG1Available sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3152 (=HL 716 + pHL 1056)
Plasmid#30048DepositorInsertpCon:MicC, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only