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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pNLF1W

Plasmid

#136404

Purpose

Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT2-Flag

Plasmid

#102623

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

Sirtuin 2 (SIRT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMEH343 CD63 MS2 HA

Plasmid

#168217

Purpose

Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

CD63 (CD63 Human)

Use

Tags

HA and MS2 dimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

FerH

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and U6

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_CAG_V5-LOV-Turbo-NES

Plasmid

#199670

Purpose

expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

NES and V5

Expression

Mutation

Promoter

CAG

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2

Plasmid

#85658

Purpose

retroviral expression of human SMYD2

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13+17

Plasmid

#61097

Purpose

Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Tito Calì, Marisa Brini

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2

Plasmid

#72595

Purpose

overexpression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
FAB(mito-LD)

Plasmid

#214425

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito

Use

Tags

CFAST-Halo and RspAN-3XHA-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-JNKKTRmRuby2

Plasmid

#59148

Purpose

ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)

Use

Tags

mRuby2

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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