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PML-3/pSG5
Plasmid#50939Purposeexpression of PML with Sumo sites destroyedDepositorInsertPML-3 (PML Human)
UseTagsHAExpressionMammalianMutationK65R, K160R, K490R (Sumo modification sites)PromoterSV40Available sinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-Q61L-shortlinker
Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_omicron_BA.1
Plasmid#180375PurposePseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike proteinDepositorInsertSpike (omicron BA.1 variant) (S SARS-CoV-2)
UseLentiviral and RetroviralTagsExpressionMammalianMutationc terminal D18PromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1
Plasmid#175766PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-CAG-CyRFP1
Plasmid#84357PurposeExpresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)DepositorInsertCyRFP1
UseAAVTagsExpressionMammalianMutationDouble FLEXedPromoterCAGAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
gag-TadCBEd
Plasmid#225144PurposePackaging plasmid for TadCBEd in v3 PE-eVLP architectureDepositorInsertMMLVgag-TadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mito-mEmerald
Plasmid#174542Purposelabels mitochondria with mEmeraldDepositorInsertMTS-mEmerald
UseLentiviralTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MSX1
Plasmid#222557PurposePiggyBac transposon plasmid for doxycycline inducible expression of MSX1DepositorInsertMSX1 (MSX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SV2A HaloTag
Plasmid#218545PurposeLentiviral expression of SV2A HaloTagDepositorInsertSV2A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-SaCas9-BlastR
Plasmid#220966PurposeExpresses human codon-optimized SaCas9DepositorInsertSaCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI SV2A pHluorin
Plasmid#218543PurposeMamallian Expression of SV2A pHluorinDepositorInsertSV2A
UseTagsExpressionMammalianMutationS42F, L199PPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRT-Fc FL
Plasmid#221853PurposeExpress human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.DepositorInsertcalreticulin (CALR Human)
UseTagsHuman IgG1Fc tagExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE FLAG-IKK2-EE puro
Plasmid#216790PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activePromoterLTRAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Dlx-FLEX-hM4Di-mCherry
Plasmid#209701PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent mannerDepositorInserthM4D(Gi)-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_AAMP:1-434
Plasmid#211192PurposeMammalian expression of full-length human AAMP. N-terminal HiBiT tag.DepositorInsertAAMP:M1-R434 (AAMP Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GluN3A/NR3A glutamate receptor [N416/57R]
Plasmid#206618PurposeMammalian Expression Plasmid of anti-GluN3A/NR3A glutamate receptor (Mouse). Derived from hybridoma N416/57.DepositorInsertanti-GluN3A/NR3A glutamate receptor (Mus musculus) recombinant Mouse monoclonal antibody (Grin3a Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
UseTagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pD649-HAsp-CD276-COMP5AP-AviTag-9xHis
Plasmid#157425PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCD276 (CD276 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
Plasmid#176396PurposeExpresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.DepositorInsertGAL1 promoter, SZ1-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_Puro_ccdb_Ubc_rtTA
Plasmid#172130PurposeDestination vector for AAVS1 targetingDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterPGK1Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SBP-GFP-Tac
Plasmid#162505PurposeExpresses GFP tagged Tac in a RUSH vector in mammalian cellsDepositorInsertTac (IL2RA Human)
UseTagsGFP and SBPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
