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pMH-SFB-BRCA1

Plasmid

#99394

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA1 (BRCA1 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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HypoxCR

Plasmid

#59946

Purpose

A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

Depositor

Anne Le

Article

Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

Inserts

GFP-Pest
mCherry-Geminin

Use

Lentiviral

Tags

FLAG and PEST

Expression

Mammalian

Mutation

Promoter

CMV and min CMV

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II-YPet(short)

Plasmid

#118714

Purpose

The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

Desmoplakin II-YPet(short) (DSP Human)

Use

Retroviral

Tags

YPet(short)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-NFE2L2-Puro

Plasmid

#181919

Purpose

Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pQHA-USP7 WT puroR

Plasmid

#46753

Purpose

Retroviral vector that expresses wild type HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Hsa

Plasmid

#128175

Purpose

Doxycyclin inducible shRNA knockdown of human cGAS gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-9

Plasmid

#208787

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 9 (CASP9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-NU

Plasmid

#121139

Purpose

Mammalian expression of GFP tagged CD274

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Inserts

CD274 (CD274 Human)
CD274 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MAVS-ts1

Plasmid

#174274

Purpose

MAVS knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MAVS shRNA (MAVS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP TMEM41B-GFP

Plasmid

#117414

Purpose

Expresses TMEM41B tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84240

Purpose

Expresses GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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