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NET4 pEGFP-N2 (1201)

Plasmid

#62041

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

Net4 (TMEM53 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
mPA-GFP-C-Src-7

Plasmid

#57127

Purpose

Localization: Membrane/Vesicles, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

C-Src

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
iLID-eGFP-3xC2

Plasmid

#114417

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

Plasmid

#107577

Purpose

B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

GFP+

Use

Synthetic Biology; E. coli and bacillus shuttle v…

Tags

B. megaterium DSM319 xylA leader sequence (MTSSKI…

Expression

Bacterial

Mutation

F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

Promoter

gapdh promoter - B. megaterium DSM319

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a V18A

Plasmid

#40054

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a V18A (SYTL2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Valine 18 to Alanine

Promoter

CMV promoter

Available since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
pRET.IL (No IRES-EGFP)

Plasmid

#1832

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pRK5 GFP RagC (GD)

Plasmid

#112767

Purpose

express GFP-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (G domain only) (RRAGC Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagB (GD)

Plasmid

#112765

Purpose

express GFP-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (G domain only) (RRAGB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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5' EGFP SNF-5 pXOON

Plasmid

#48700

Purpose

Encodes 5' EGFP - SNF-5 fusion construct for expression in Xenopus oocytes or Mammalian Cells

Depositor

Dmitri Boudko

Insert

snf-5 (snf-5 Nematode)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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PKAcs-KR6X-GFP

Plasmid

#108585

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with structured linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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PKAcs-L10X-GFP

Plasmid

#108587

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with structured linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCCr4

Plasmid

#79252

Purpose

Expresses EGFP-tagged DmCCr4 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmCaf40 (twin Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt Fly, NM_164598)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBS593 promoterless GFPcre

Plasmid

#11959

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-ARAP3(R1155E)

Plasmid

#39488

Depositor

Sonja Vermeren

Article

Krugmann et al Curr Biol. 2004 Aug 10;14(15):1380-4.

Insert

ARAP3(R1155E) (ARAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R1155E = Ras binding domain mutation

Promoter

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-ARAP3(C504A)

Plasmid

#39485

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3(C504A) (ARAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C504A = Arf GAP domain mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
Gaq-LSS-SGFP2

Plasmid

#112951

Purpose

Galphaq tagged with LSS-SGFP2

Depositor

Dorus Gadella

Insert

Galphaq

Use

Tags

LSS-SGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-152-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85872

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-152-3p Sensor (MIR152 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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