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Plasmid#40388DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2186, C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits
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pBACgus_Sumostar/LRRK2 1867-2161_G2019S
Plasmid#40393DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161, G2019SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-VEE-IRES-Puro
Plasmid#58970Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorInsertXRCC4 (XRCC4 Human)
UseTagsGFP-2xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
AIO-GFP
Plasmid#74119PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R-6His
Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
UseTagsHisExpressionBacterialMutationPromoterT7/Sp6Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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SNAP-23
Plasmid#186686PurposeExpresses SNAP-23 in E. ColiDepositorInsertSNAP23
UseTagsGST and thrombin siteExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrEno
Plasmid#115474PurposeEno-promoter driven expression vector for protein production in Trichoderma reeseiDepositorInsertCel7A
UseUnspecifiedTagsExpressionMutationIntrons have been removedPromoterTrichoderma reesei Enolase (Eno) promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFV2-HA
Plasmid#187082PurposeExpression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)DepositorInsertatpBEFHAGDC
UseTags6-His beta subunit (atpD) and HA tag subunit a (a…ExpressionBacterialMutationAll Cys codons changed to Ala.PromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS9counter
Plasmid#107192PurposeExpresses CAS9 and sgRNA for targeted counterselection in S. aureusDepositorInsertsCas9
sgRNA
UseTagsExpressionBacterialMutationPromoterprab17Available sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version1
Plasmid#197931PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTagsFLAG tag and SV40 NLS and nucleoplasmin NLSExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP)
Plasmid#102425PurposeHA tag fused to the N-terminus of RILP for the expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
UseTagsHAExpressionMammalianMutationPromoterTet-responsiveAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (TF)
Plasmid#92213PurposeTranscription factor component of FLAREDepositorInsertNRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only