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Plasmid#12188DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationKinase dead: K1361MPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha WT GFP
Plasmid#128819PurposeTo visualize Doc2alpha WT in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rac1 QL
Plasmid#61633Purposemammalian expression of rac1 constitutively active mutantDepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationQ61L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7b1(glu)
Plasmid#14673DepositorInsertMKK7b1(Glu) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationSer-269Glu Thr-273GluPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPW3763 : CMVd3-HsIRE1dLKR-HaloTag
Plasmid#185685PurposeLow-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationDeletion of linker, kinase, and Rnase domainsPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-mIR-31 sponge
Plasmid#22694PurposeStable suppression of miR-31 functionDepositorInsertmiR-31 (MIR31 Human)
UseRetroviralTags*ExpressionMammalianMutationmiR-31 sponge with 7x tandem binding sites to the…PromoterAvailable sinceMarch 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertFKBP-ICUE3-NLS
UseTagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-E257A
Plasmid#31727DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged E257 to AlaninePromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
YFP-Stim1-D76A
Plasmid#186622PurposeEncodes Stim1 mutant D76A fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationD76APromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Y587E EphA2 pcDNA3
Plasmid#102730PurposeExpression of EphA2 in mammalian cellsDepositorInsertEphA2 (Epha2 Mouse)
UseTagsExpressionMammalianMutationY587E (TAT > GAG)PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIK208
Plasmid#65630PurposesgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"DepositorInsertU6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…TagsExpressionMutationG174DPromoterPelcAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA
Plasmid#187063PurposeNpu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertNpu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-S259A
Plasmid#31728DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutationChanged S259 to AlaninePromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only