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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
pAG305GPD-ccdB-EGFP

Plasmid

#14186

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 EGFP-guide2

Plasmid

#118167

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-STAG2-S653Stop

Plasmid

#31976

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

changed Serine 653 to Stop codon

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-alpha1-chimaerin

Plasmid

#59317

Purpose

Expression plasmid for GFP-tagged alpha1-chimaerin (GFP at N-terminus of alpha1-chimaerin)

Depositor

Peter Scheiffele

Article

Beg et al Neuron. 2007 Sep 6;55(5):768-78.

Insert

alpha1-chimaerin

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Δ741-752-GFP

Plasmid

#33119

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of aa 741-752

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCaSpeR3/ncd-gfp #10

Plasmid

#66082

Purpose

stable germline transformation of Drosophila to produce Ncd-GFP expression

Depositor

Sharyn Endow

Article

Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

Insert

Ncd kinesin-14 motor protein (ncd Fly)

Use

Tags

GFP

Expression

Insect

Mutation

D699L

Promoter

ncd promoter (3.1kb upstream region of ncd start …

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Promoter

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMAM-RecG-EGFP

Plasmid

#31274

Depositor

Andrew Pierce

Article

Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

Insert

RecG-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pAG304GPD-ccdB-EGFP

Plasmid

#14184

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha270-1374delNLS2

Plasmid

#62532

Purpose

N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and nuclear localizatio…

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
CS2-H2B-EGFP

Plasmid

#132414

Purpose

To make synthetic mRNA encoding a nuclear EGFP

Depositor

David Kimelman

Insert

H2B-EGFP (H2BC21 Synthetic)

Use

Synthetic mrna production

Tags

Expression

Mutation

Promoter

SP6

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-RCY1

Plasmid

#105281

Purpose

Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Rcy1 (RCY1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-ArchT-GFP

Plasmid

#86536

Purpose

Retrovirus expressing optogenetic silencer ArchT-GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

ArchT

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Use

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-UVRAG S498D

Plasmid

#86742

Purpose

N-terminal EGFP-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S498D

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAG305GPD-EGFP-ccdB

Plasmid

#14306

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV2-OCT-HA-eGFP

Plasmid

#67481

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only