Skip to main content
Addgene
Showing: 6121 - 6140 of 30551 results

  1. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid
    #101185
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    G582A/V583A/E584A/D585A/N588A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101185-plasmid-map-sequence-id-194249

    Enlarge

  2. MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A

    Plasmid
    #101179
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101179-plasmid-map-sequence-id-194003

    Enlarge

  3. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid
    #101180
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101180-plasmid-map-sequence-id-194005

    Enlarge

  5. pZFN-AAVS1-KKR

    Plasmid
    #159298
    Purpose
    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    ZFN targeting the AAVS1 locus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159298-plasmid-map-sequence-id-311594

    Enlarge

  6. CRY2olig-NONO

    Plasmid
    #218922
    Purpose
    Expression of CRY2olig-mCherry fused to NONO
    Depositor
    Tatyana Shelkovnikova
    Insert
    CRY2olig-mCherry tagged NONO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218922-plasmid-map-sequence-id-442216

    Enlarge

  7. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

    Enlarge

  8. pLenti X1 Zeo/pTER shMDC1 (673-1)

    Plasmid
    #22193
    Depositor
    Eric Campeau
    Insert
    sh Mediator of DNA damage checkpoint 1
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 18, 2009
    Availability
    Academic Institutions and Nonprofits only
    22193-plasmid-map-sequence-id-10169

    Enlarge

  9. pmCherry Paxillin

    Plasmid
    #50526
    Depositor
    Kenneth Yamada
    Insert
    PXN paxillin (PXN Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50526-plasmid-map-sequence-id-241928

    Enlarge

  10. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

    Enlarge

  11. pLenti-TRE-GFP-Blast

    Plasmid
    #135673
    Purpose
    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135673-plasmid-map-sequence-id-327577

    Enlarge

  12. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

    Enlarge

  13. pCre-P2A-mCherry

    Plasmid
    #212105
    Purpose
    Expression of Cre and mCherry in mammalian cells
    Depositor
    Daekee Lee
    Insert
    Cre recombinase and mCherry
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212105-plasmid-map-sequence-id-419563

    Enlarge

  14. mCherry-FAK-5

    Plasmid
    #55044
    Purpose
    Localization: Focal adhesion kinase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    FAK
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55044-plasmid-map-sequence-id-218555

    Enlarge

  15. RHPMP139 (pLifeAct mEOS3.1)

    Plasmid
    #88839
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    mEOS3.1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88839-plasmid-map-sequence-id-177489

    Enlarge

  16. RHPMP140 (pLifeAct mOrange2)

    Plasmid
    #88840
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88840-plasmid-map-sequence-id-177491

    Enlarge

  17. EBFP2-Lysosomes-20

    Plasmid
    #55246
    Purpose
    Localization: Lysosome Membrane, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. sfGFP-Lysosomes-20

    Plasmid
    #56487
    Purpose
    Localization: Lysosome Membrane, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56487-plasmid-map-sequence-id-456534

    Enlarge

  19. pCDH-EF1s

    Plasmid
    #72484
    Purpose
    Express gene of interest under truncated EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72484-plasmid-map-sequence-id-134994

    Enlarge

  20. pLPC-N FLAG

    Plasmid
    #12521
    Depositor
    Titia de Lange
    Insert
    N FLAG
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2006
    Availability
    Academic Institutions and Nonprofits only
    12521-plasmid-map-sequence-id-123475

    Enlarge

Showing: 6121 - 6140 of 30551 results