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  7. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid
    #216124
    Purpose
    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216124-plasmid-map-sequence-id-428729

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  10. pUD-MSP1D1

    Plasmid
    #161608
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans and tyrosines w…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161608-plasmid-map-sequence-id-317655

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Showing: 3061 - 3070 of 148463 results