Addgene

We narrowed to 51,918 results for: cloning

Showing: 6101 - 6120 of 51918 results

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pQTEV-TPT1

Plasmid

#31558

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

tumor protein, translationally-controlled 1 (TPT1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A3

Plasmid

#132024

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A3 (SLC22A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBEG R2-L3 #BGV193

Plasmid

#48952

Purpose

For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pJOG022

Plasmid

#105384

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor Zea mays Ubiquitin (1500bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-PTD004

Plasmid

#34798

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

GTP-binding protein PTD004 (OLA1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

Plasmid

#13006

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 deltaCC (SULF2 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Cysteine 88 to Alanine

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPA14

Plasmid

#19542

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 14 (HSPA14 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_5_neg_Control

Plasmid

#130558

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-1.1-1.10a

Plasmid

#69589

Purpose

OT-II TCR a chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR alpha clone 1.1 (Tcra Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pJOG140

Plasmid

#105399

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

PKI nuclear export signal

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG141

Plasmid

#105400

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

SV40 nuclear localization signal

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA1

Plasmid

#19464

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJA1 (DNAJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

D244G

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA2

Plasmid

#19465

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ subfamily A member 2 (DNAJA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Use

Tags

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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