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Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
UseTagsExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-plap
Plasmid#80422Purposetracing neuronal projectionDepositorInsertplap (ALPP Human)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25F
Plasmid#91143PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromotertacAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-SNF7-mCherry (SNF7 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
APEX2-GBP
Plasmid#67651PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-pEF
Plasmid#49446PurposeThis plasmid expresses full-length human TET3.DepositorInsertTet methylcytosine dioxygenase 3 (TET3 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-4GS-LaG-2
Plasmid#172764PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)DepositorInsertLaG-16-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-TSG101
Plasmid#116925Purposeexpresses mEGFP-TSG101 in mammalian cellsDepositorInsertTSG101 (TSG101 Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-dADAM17 (#1)
Plasmid#177259PurposeA knockout vector for the dog Adam17DepositorInsertA gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM82
Plasmid#133648PurposeIntegration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mCherry (NAB2 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-ZIP-GFP11
Plasmid#182243PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)DepositorInsertGFP10-ZIP-11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-Flag-RNF169
Plasmid#74243PurposeExpresses RNF169 in mammalian cellsDepositorInsertRNF169 (RNF169 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZJOM119
Plasmid#133661PurposeIntegration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.DepositorInsertp1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only