Showing: 6081 - 6100 of 6450 results
-
Plasmid#147213PurposeInsect Expression of DmXRN1-W1345A-dsRNAresDepositorInsertDmXRN1-W1345A-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailabilityAcademic Institutions and Nonprofits only -
ACPPiso1-V5
Plasmid#113052PurposeExpresses human prostatic acid phosphatase (isoform 1) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 1 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ACPPiso2-V5
Plasmid#113053PurposeExpresses human prostatic acid phosphatase (isoform 2) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 2 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX-FLAG-CH3 Del33
Plasmid#133724PurposeRetroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlagExpressionMutationpartial (not complete) p300 fragment, AT and CH3 …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
VPS4B, S2C
Plasmid#184806PurposeBacterial expression of VPS4B (S2C mutation)DepositorInsertVPS4B (VPS4B Human)
UseTagsHis6ExpressionBacterialMutationS2CPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
yPet_cp229-Binder
Plasmid#179137PurposeYPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).DepositorInsertSspB
UseTagsyPet_cp229ExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailabilityAcademic Institutions and Nonprofits only -
Flag-mCherry-Binder
Plasmid#179139PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailabilityAcademic Institutions and Nonprofits only -
SnapTag-Binder
Plasmid#179142PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsSNAP-tagExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-CFL-SN
Plasmid#181740PurposeAAV vector for expressing CFL-SNDepositorInsertCFL-SN
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f
Plasmid#181742PurposeAAV vector for expressing CFL-SN and GCaMP6fDepositorInsertCFL-SN-P2A-GCaMP6f
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
AA403
Plasmid#215960PurposeFragmid fragment: (guide cassette) guide expressionDepositorInsertU6_v1; DR_v0 [Cas7-11]; BsmBI_v16; BsmBI_v12
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Src(KD/YF)-SsrA(Y7I)-mCer3
Plasmid#221000Purposethis is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminalDepositorInsertSrc, SsrA, mCerulean3 (Src Mouse)
UseTagsmCerulean3ExpressionMammalianMutationD388R/Y529F in Src, Y7I in SsrAPromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2ΔC990
Plasmid#66946PurposeMammalian expression of rat ErbB2 ΔC990 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutationDeletion of C990. (see depositor comment below on…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2ΔC776
Plasmid#66947PurposeMammalian expression of rat ErbB2 ΔC776 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutationDeletion of C776 (see depositor comment below on …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2
Plasmid#66948PurposeMammalian expression of rat ErbB2 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutation(See depositor comment below on mutations A24T an…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.BRWD3-V5H
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6
Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 6081 - 6100 of 6450 results