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Addgene
Showing: 6081 - 6100 of 16015 results

  1. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  2. pCMV4a-hFJX1-Flag

    Plasmid
    #46931
    Purpose
    expression of human FJX1-FLAG in mammalian cells
    Depositor
    R.D. Beauchamp, Natasha Deane
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  3. APEX2 VLP

    Plasmid
    #182211
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Availability
    Academic Institutions and Nonprofits only
    182211-plasmid-map-sequence-id-357716

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  4. Akaluc VLP

    Plasmid
    #182212
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-Akaluc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Availability
    Academic Institutions and Nonprofits only
    182212-plasmid-map-sequence-id-357717

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  5. meGFP-hTRAK1

    Plasmid
    #188664
    Purpose
    human TRAK1 with N-terminal meGFP tag
    Depositor
    Thomas Schwarz
    Insert
    OIP106 (TRAK1 Human)
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    188664-plasmid-map-sequence-id-369503

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  6. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  7. TBRII-C-18

    Plasmid
    #62797
    Purpose
    Localization: Membrane Receptor, C1 Cloning Vector, Excitation:N/A, Emission:N/A
    Depositor
    Michael Davidson
    Insert
    TBRII (TGFBR2 Human)
    Use
    Tags
    SNAP Tag
    Expression
    Mammalian
    Mutation
    H385N
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    62797-plasmid-map-sequence-id-173039

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  8. pLVX-EGFP-C3-cenexin

    Plasmid
    #73334
    Purpose
    Lentiviral vector encoding cenexin with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    cenexin/ODF2 (ODF2 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    73334-plasmid-map-sequence-id-139040

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  9. pCMVHA-E2F5

    Plasmid
    #24213
    Purpose
    Mammalian expression of human E2F5 with HA tag
    Depositor
    Kristian Helin
    Insert
    E2F5 (E2F5 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24213-plasmid-map-sequence-id-245679

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  10. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  11. pJK402

    Plasmid
    #72415
    Purpose
    Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72415-plasmid-map-sequence-id-135527

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  12. pJK523

    Plasmid
    #72442
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72442-plasmid-map-sequence-id-133863

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  13. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  14. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  15. pJK532

    Plasmid
    #72513
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72513-plasmid-map-sequence-id-132539

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  16. pJK620

    Plasmid
    #72537
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72537-plasmid-map-sequence-id-135983

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  17. pJK622

    Plasmid
    #72538
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    72538-plasmid-map-sequence-id-132629

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  18. sfCherry11-Ex1-17-PTPN22

    Plasmid
    #195387
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17) with sfCherry(11) tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    sfCherry(11)
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195387-plasmid-map-sequence-id-387974

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  19. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

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Showing: 6081 - 6100 of 16015 results