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cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha-pcDNA3.EGFP

Plasmid

#13009

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Bmal1

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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Inducible Caspex expression

Plasmid

#97421

Purpose

For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

Depositor

Samuel Myers, Steven Carr

Article

Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

Inserts

Caspex
T2A-Gfp

Use

CRISPR

Tags

3xFLAG and V5

Expression

Mammalian

Mutation

dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

Promoter

TRE3G

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-EYFP

Plasmid

#34582

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Type

Empty backbone

Use

Tags

3xFLAG and EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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