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Plasmid#120513PurposeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Thr58Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Threonine to Alanine at a…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-MOR-FRB
Plasmid#191497PurposeRapamycin-dependent opioid sensor for the mu opioid receptor (component 1)DepositorInsertMOR-FRB
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTALEN(Nd154C63) MND
Plasmid#50432PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterMNDAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2304
Plasmid#142020PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCIITA (CIITA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.NWS.VSVg_NGFR
Plasmid#158243PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.NWS.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MARK4 gRNA (BRDN0001145462)
Plasmid#76727Purpose3rd generation lentiviral gRNA plasmid targeting human MARK4DepositorInsertMARK4 (MARK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/vpx
Plasmid#115808PurposeSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpxDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti6.3/hCx43-stop
Plasmid#27383DepositorInsertGja1 (GJA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
shLacZ
Plasmid#42559DepositorInsertshLacZ
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA-SHLD2
Plasmid#114121PurposeLentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatinDepositorInsertSHLD2 (SHLD2 Human)
UseLentiviral; Doxycycline inducibleTagsRNF8 FHA domain (aa 2-160) and eGFPExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-DBNF-mCherry
Plasmid#187195PurposeTo express the mouse BDNF tagged with the FP mCherryDepositorInsertBDNF
UseLentiviralTagsfluorescent protein mCherryExpressionMammalianMutationThe codons have been optimized for better express…PromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgAAVS1
Plasmid#187818PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pf CAG mc2 IRES puro
Plasmid#206233PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-SIRPB1
Plasmid#116790PurposeLentiviral expression of SIRPB1DepositorInsertSIRPB1 (SIRPB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE
Plasmid#140228PurposeRRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.DepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseLentiviralTagsBFPExpressionMammalianMutationPromoterRRM2 endogenous promoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188771PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEFSAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST
Plasmid#164229Purposelentiviral plasmid for GFP-hTREX1 delta C (aa1-235)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeleted amino acids 235-314PromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-Hygro
Plasmid#190805PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance geneDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationSwedish (KM670/671NL)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MARK3 gRNA (BRDN0001146658)
Plasmid#77719Purpose3rd generation lentiviral gRNA plasmid targeting human MARK3DepositorInsertMARK3 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorInsertmfn2 (MFN2 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only