-
Plasmid#202422PurposeExpression of GFP-tagged PODXL without intracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted intracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FBM
Plasmid#202417PurposeExpression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)DepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationH477/R479/S481>AAA mutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 C-V5-tag ORF7a
Plasmid#179971PurposeMammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 ORF7a (ORF7a Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP7 C-V5-tag
Plasmid#179963PurposeMammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp7 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.444Gwt
Plasmid#190476Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Awt
Plasmid#190478Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITGA5_1745G>U
Plasmid#173812Purposemir218 binding site2 point mutationDepositorInsertNT1745 ITGA5 point mutation (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAmpRAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITGA5_473G>A
Plasmid#173811Purposemir218 binding site1 point mutationDepositorInsertNT473 ITGA5 point mutation (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAmpRAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR218-2_nt5G>U
Plasmid#174009PurposemiR218 nt5 point mutation in SLIT3DepositorUseTagsExpressionMammalianMutationNT5 G>U point mutation in SLIT3PromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITGA5_Q853L
Plasmid#174008PurposeFibronectin receptor binding site point mutationDepositorInsertFibronR ITGA5 point mutation (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAmpRAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shAtxn1
Plasmid#180387PurposeProducing AAV that encodes mouse Atxn1 shRNA with miR-E backboneDepositorInsertshAtxn1 (Atxn1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 ORF7a C-V5-tag
Plasmid#179989PurposeMammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 ORF7a
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 NSP16 C-V5-tag
Plasmid#179985PurposeMammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 Nsp16
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 ORF6 C-V5-tag
Plasmid#179970PurposeMammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 ORF6 (ORF6 Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 E C-V5-tag
Plasmid#179966PurposeMammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 E (E Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP16 C-V5-tag
Plasmid#179965PurposeMammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp16 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-AAFF
Plasmid#171218PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.DepositorInsertAR-AAFF (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K580A-V581APromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry
Plasmid#172638PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationThr286IlePromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CCND1(P287A)-Puro
Plasmid#172641PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1(P287A) and a puromycin resistance marker in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPro287AlaPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only