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Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRU5-inmCherry
Plasmid#60238PurposeHTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(+)-Fc
Plasmid#59314PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]DepositorInsertNrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
UseTagsFc (from human IgG)ExpressionMammalianMutationPromoterCMV, T7Available sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD280.7
Plasmid#173172PurposeExpresses SO_4387DepositorInsertSO_4387
UseTagsExpressionBacterialMutationPromoterJ23107Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF152 pcDNA3.1(+)SOD1A4V
Plasmid#26398DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationA4VPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
Plasmid#46676DepositorInserthsa-mir-133a-1 (MIR133A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2-CAG-fGFP
Plasmid#108714PurposeTransposon donor plasmid for long-term expression of membrane targetted GFPDepositorInsertEGFP
UseTags6xHis and farnesylation signalExpressionMammalianMutationPromoterCAGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT40
Plasmid#80415PurposeExpresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1.Tat(48-57)-Cre recombinase
Plasmid#165042PurposeExpression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)DepositorInsertCre recombinase
UseTagsMxe Intein-Chitin binding domain and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJG85
Plasmid#89281PurposeEntry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sitesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES EGFP
Plasmid#130706PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
∆C' terminal-DPYSL2-pLJC2
Plasmid#189867PurposeExpresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cellsDepositorInsertΔC-DPYSL2
UseLentiviralTags3X FLAGExpressionMammalianMutationdeleted the C'terminus of DPYSL2 (491-572 aa)PromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sf-U7snRNA-ESE-AS-NsiI
Plasmid#190694PurposeBasic vector used for the expression of U7-based antisense oligonucleotidesDepositorInsertU7 snRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Vinculin-23
Plasmid#55516PurposeLocalization: Focal Adhesions, Excitation: 462, Emission: 492DepositorInsertVinculin (VCL Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pCR3.1)
Plasmid#26573DepositorInsertCacna1a (Cacna1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only