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Plasmid#188472PurposescDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFPDepositorInserteGFP-WPRE-DAISY-4T
UseTagsExpressionMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip
Plasmid#178054Purposemyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsmycBioIDExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAK184
Plasmid#167281PurposeMazF-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterPxylAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1 sgTollip
Plasmid#196546PurposeLentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire-NRN
Plasmid#149692PurposeFor packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE
Plasmid#138145PurposeExpresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.DepositorInsertPspCas13b
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Clathrin-15
Plasmid#58005PurposeLocalization: Clathrin Vesicles, Excitation: 555, Emission: 584DepositorInsertClathrin (CLTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre
Plasmid#203839PurposeFlp-dependent enzymatically inactive Cre and mScarlet-KASH expressionDepositorInsertdCre
UseAAVTagsmScarlet-KASH-2AExpressionMutationEnzymatically inactive CrePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EBFP2-P2A-EGFP
Plasmid#184938PurposeExpresses blue fluorescent protein EBFP2 and EGFP in mammalian cellsDepositorInsertEBFP2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCMV-H2B-FT-Slow
Plasmid#157671PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsSlow-FTExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SuExp-hPLD3
Plasmid#201243Purposeexpress full length human PLD3 proteinDepositorInsertPLD3 (PLD3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
alpha-0 CFP in pcDNA3.1
Plasmid#36396DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationCFP inserted into the KpnI-SpeI sites engineered …PromotercmvAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-222PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCMV-H2B-FT-Fast
Plasmid#157669PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsFast-FTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Snail
Plasmid#23347DepositorInsertSnail (SNAI1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATXN1-30Q
Plasmid#33238DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGFPExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MCU-V5-HIS
Plasmid#31731DepositorInsertMCU (MCU Human)
UseTagsV5-HIS6XExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAmCyan1-C1-ADCY6
Plasmid#113905PurposeN-terminal Cyan Fluorescent tag in Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 (ADCY6 Human)
UseTagsCyanExpressionBacterial and MammalianMutationPromoterpCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Caveolin-3
Plasmid#68396PurposeFluorescently tagged Caveolin-3 for expression in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only