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Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30RPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7 (hspb7 Zebrafish)
UseTagsHalo-TEVExpressionMammalianMutationPromoterSP6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS
Plasmid#158739PurposeExpresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFPDepositorInsertHemagglutinin A/TX/50/12 H3
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationG186V S219FPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZC13 (pFA6a-TEV-6xGly-V5-HphMX)
Plasmid#44085DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-CaM-NanoLuc-15aa-uTEVp
Plasmid#163034PurposeFLiCRE protease with luciferin-gating for neuronal expressionDepositorInsertGFP-CaM-NanoLuc-15aa-uTEVp
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
Plasmid#135458PurposeMammalian expression of SPARK TF componentDepositorInsertβ2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5
UseLentiviralTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL4 CTF-FLAG
Plasmid#217709PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL4 (ADGRL4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-406PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Tev_ACTA1 (alpha-actin)
Plasmid#188452PurposeExpress in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.DepositorInsertACTA1 (ACTA1 Human)
UseTags6xHis-FLAGExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV2 TEV-NSP3
Plasmid#154402PurposeGateway-compatible Entry vectorDepositorInsertNSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
Plasmid#137830PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationMutated Leucine 71 to Lysine in CTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03
Plasmid#158793PurposeExpresses influenza A virus tetrameric neuraminidase fused to a sfGFPDepositorInsertNeuraminidase A/NL/109/03
UseTagsTwinStrep_sfGFP_tetrabrachionExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX-(3×FLAG)-TEV-ProtA
Plasmid#52693PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-FKBP-Myc
Plasmid#177908Purposehuman CD81-TEV cleavage site-FKBP-Myc tagDepositorInserthuman CD81 (CD81 Synthetic, Human)
UseTagsFKBP, Myc tag, and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170995PurposeHiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)DepositorInsertAKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-proCaspase-3-TEVp
Plasmid#166607PurposeEncodes Cre-dependent procaspase 3 and TEVp under control of the TREDepositorInsertprocaspase3- TEVp
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-Twin-Strep
Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
Plasmid#217708PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL3 (ADGRL3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-841PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB1 CTF-FLAG
Plasmid#217681PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB1 (ADGRB1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-926PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only