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pAN-PTet-dCas9

Plasmid

#62244

Purpose

controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

dCas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

catalytically inactive, D10A, H840A

Promoter

Ptet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Wt1

Plasmid

#41082

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Wt1 (-KTS alternative isoform) (Wt1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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TetO-dCas9-D3A

Plasmid

#78254

Purpose

Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-tet

Plasmid

#172214

Purpose

For allelic exchange in tetracyclin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Tags

Expression

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Nfil3

Plasmid

#139815

Purpose

doxycycline-inducible expression of mouse Nfil3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfil3 (Nfil3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ascl2

Plasmid

#71151

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ascl2 (Ascl2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX17FNV

Plasmid

#206384

Purpose

Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX17FNV (SOX17 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FGL

Plasmid

#190234

Purpose

AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

LDLR C-terminal sequence and myristoylation signa…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Sox10

Plasmid

#45843

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep152

Plasmid

#136874

Purpose

Mammalian expression of the centrosomal protein Cep152 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep152 (CEP152 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk12_Untagged

Plasmid

#127178

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Mutation

Promoter

TetO Promoter

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT-YFP-TetR

Plasmid

#59018

Purpose

Expresses a fusion of yellow ﬂuorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

Depositor

David Sibley

Article

Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

Insert

YFP-TetR

Use

Toxoplasma expression

Tags

Expression

Mutation

Promoter

alpha-tubulin

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Cebpa

Plasmid

#139834

Purpose

doxycycline-inducible expression of mouse Cebpa in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Cebpa (Cebpa Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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epB_CAG_TetRFlag-nls-tdTom_DEx4

Plasmid

#119909

Purpose

ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-tdTom

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 HxD

Plasmid

#136365

Purpose

Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

HxD (H1077Y, D1079A)

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Minimal CMV + TetO

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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TetR in pENTR1A (559-1)

Plasmid

#22265

Depositor

Eric Campeau

Insert

Tetracycline repressor (tetR )

Use

Tags

Expression

Insect, Mammalian, Worm Exp…

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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