Addgene

Showing: 581 - 600 of 1400 results

Results per page:

Sort results by:

pSDMA25

Plasmid

#67940

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)

Depositor

James Fraser

Article

Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

Type

Empty backbone

Use

Cryptococcal complementation vector

Tags

Expression

Mutation

Promoter

ACT1 for Cryptococcal marker

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG1.1

Plasmid

#173685

Purpose

The expression vector for genes of interest under CAG promoter.

Depositor

Masahito Ikawa

Article

Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Pdx1 Cre (DM#258)

Plasmid

#15021

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TarHom-Tol2-kanaR-cryaa-dsRed

Plasmid

#74152

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-dsRed

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TarHom-Tol2-kanaR-cryaa-CFP

Plasmid

#74151

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-CFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDMA58

Plasmid

#67942

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.

Depositor

James Fraser

Article

Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

Type

Empty backbone

Use

Cryptococcal complementation vector

Tags

Expression

Mutation

Promoter

ACT1 for Cryptococcal marker

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSB-609 U6-sgRNA1 ATCC GGAA

Plasmid

#173671

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSB-610 U6-sgRNA2 GGAA AGAG

Plasmid

#173672

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSB-611U6-sgRNA3 AGAG AACA

Plasmid

#173673

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK394 (mAID-Nluc-Neo)

Plasmid

#121195

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc-Neo

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-fSNAP-URA

Plasmid

#188917

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-URA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

K1750A

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-U6

Plasmid

#120358

Purpose

piggyBac transposon vector for sgRNA expression.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
BAX KO vector

Plasmid

#16575

Depositor

Bert Vogelstein

Article

Zhang et al Science. 2000 Nov 3. 290(5493):989-92.

Insert

BAX knockout (BAX Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
foxn4

Plasmid

#11260

Depositor

Lisa Steiner

Article

Danilova et al Brain Res Dev Brain Res. 2004 Oct 15. 15

Insert

foxn4 (foxn4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge