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Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-T7-Ct-YhjH
Plasmid#79167PurposeDual expression of Ct biosensor and YhjH under individual T7 promotersDepositorInsertsyhjH
T7-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-hTDG
Plasmid#70758Purposebacterial expression of human TDGDepositorInsertTDG (TDG Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28HistevTtTERT(1-516)
Plasmid#28171DepositorInsertTetrahymena TERT
UseTags6xhistidineExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS1
Plasmid#90027PurposeTo express His-tagged CAHS1 in bacteriaDepositorInsertCAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEThT-CAHS3
Plasmid#90028PurposeTo express His-tagged CAHS3 in bacteriaDepositorInsertCAHS3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-At-TRL
Plasmid#32242DepositorInsertA. thaliana tRNA ligase (RNL Mustard Weed)
UseTags6xHistidineExpressionBacterialMutationPromoterT7 RNA PolmyeraseAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP2
Plasmid#71466PurposeEncodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 2 (SENP2 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS2
Plasmid#90026PurposeTo express His-tagged SAHS2 in bacteriaDepositorInsertSAHS2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
UseTagsGSTExpressionBacterialMutationS72TPromoterT7Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
BglB in pET29b(+)
Plasmid#55773PurposeExpresses beta-glucosidaseDepositorInsertBeta-glucosidase B (bglB Synthetic)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
UseTags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertMTdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIE
Plasmid#171083PurposeCo-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_AQUAR
Plasmid#204239PurposeBacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_AQUAR
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only