Addgene

We narrowed to 1,050 results for: mcp

Showing: 581 - 600 of 1050 results

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-STIL

Plasmid

#80265

Purpose

baculovirus creation plasmid for GST-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al Elife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Use

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-100

Plasmid

#47771

Purpose

expresses AmTrac-100 in yeast

Depositor

Wolf Frommer

Article

De Michele et al Elife. 2013 Jul 2;2:e00800.

Insert

AmTrac-100

Use

Tags

mcpGFP

Expression

Yeast

Mutation

A141E, T464D

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Use

Tags

3xFLAG and NLS

Expression

Mutation

Promoter

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ALIX-mNeonGreen

Plasmid

#191191

Purpose

Full-length ALIX with C-terminal mNeonGreen tag.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra2-LaminB1-10

Plasmid

#57728

Purpose

Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LaminB1 (LMNB1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ALIX-His(1-868, P801G)

Plasmid

#186808

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

ALIX-His (1-868, P801G) (PDCD6IP Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Proline 801 to Glycine

Promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL 5A

Plasmid

#80267

Purpose

mammalian expression plasmid for MycLAP-STIL 5A

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

S1103A, S1108A, S1111A, S1116A, T1119A

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-NLS-tdPCP-EGFP

Plasmid

#99910

Purpose

For the expression of NLS-tPMCP-EGFP

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-tdPCP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE4G

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Cdk6

Plasmid

#1866

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA6.MCV.LT339.V5.4

Plasmid

#28193

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCPyV Truncated Tumor Large T antigen (399 tumor strain)

Use

Tags

V5

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pLX302-PTP-SL-V5 puro

Plasmid

#87774

Purpose

Expresses V5 tagged human PTP-SL

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

PTP-SL (PTPRR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302-NIPP1-V5 puro

Plasmid

#87775

Purpose

Expresses V5 tagged human NIPP1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

NIPP1 (PPP1R8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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