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pFS_0349_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1

Plasmid

#116961

Purpose

Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC

Plasmid

#116962

Purpose

Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC

Plasmid

#116990

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1

Plasmid

#116987

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0392_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2_RC

Plasmid

#117004

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0391_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2

Plasmid

#117003

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0390_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1_RC

Plasmid

#117002

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0389_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1

Plasmid

#117001

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC

Plasmid

#116986

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC

Plasmid

#116984

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1

Plasmid

#116985

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC

Plasmid

#116982

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2

Plasmid

#116983

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-cTEV-PuroR

Plasmid

#80958

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

c-terminal split TEV

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-nTEV-PuroR

Plasmid

#80963

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

n-terminal split TEV

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 RRN3

Plasmid

#67112

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RRN3 (RRN3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TCEB3B

Plasmid

#67117

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCEB3B (ELOA2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFAP4

Plasmid

#67073

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP4 (TFAP4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

P338A

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
XP31 Vox

Plasmid

#16972

Depositor

Randall Moon

Article

Schmidt et al Development. 1996 Jun;122(6):1711-21. do

Insert

Vox (ventx2.1.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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