-
Plasmid#55592PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertCFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-stuffer (infusion)-IRES-CFP
Plasmid#114727Purposeexpression in mammalian cellsDepositorInsertstuffer (infusion)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP-e2a-SOX2-p2a-KLF4
Plasmid#193358PurposeVEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)DepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti sgRNA NGFR CFP out of frame
Plasmid#155283PurposeLentiviral plasmid for sgRNA, NGFR marker, editing detected with CFP, used with 155280DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)Stop in pcDNA1/amp
Plasmid#60893PurposeThis vector attached CFP(159-238) to the C-terminus of a protein.DepositorInsertCFP(159-238)Stop
UseTagsExpressionMammalianMutationAsn-164 was changed to HisPromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#55777PurposeA C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.DepositorInsertCFP(159-238)-gamma-2 (GNG2 Human, Aequorea victoria)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)
Plasmid#14850DepositorInsertGAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
Plasmid#158654Purposemaking transgenics expressing a QUAS driven YFP and NTR 2.0DepositorInsert5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
α-catenin ECFP-YPET FRET ΔF-actin binding site
Plasmid#198245PurposeAlpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.DepositorInsertα-catenin ECFP-YPET FRET sensor ΔF-actin binding site
UseTagsECFP and YPETExpressionMammalianMutationDeleted F-actin binding site on a-catenin (849-90…PromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066-ECFP FRET
Plasmid#46179DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP, EYFP, and HisExpressionBacterialMutationaa 884-1066 (Vt, tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
LZF1827 pAAV_hSyn-Flp-off-Cre-on-stGtACR2-CFP
Plasmid#135413Purposeexpresses GtACR2 linked to CFPDepositorInsertstGtACR2-CFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZF39: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104118PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104119PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseAdenoviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP
Plasmid#114729Purpose3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M5, sgRNA M7, sgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP
Plasmid#73200PurposeBAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker CeruleanDepositorInsertCFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -