Addgene

Showing: 581 - 600 of 11035 results

Results per page:

Sort results by:

pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

Plasmid

#209775

Purpose

CRISPRi for STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shId2 #2

Plasmid

#83090

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 SARS-CoV-2 S D614

Plasmid

#158074

Purpose

Expresses SARS-CoV-2 spike protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FH1-MGT#2-mCherry_PGK-H2B-CFP

Plasmid

#164102

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

D614G

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-S2P-AVI

Plasmid

#160474

Purpose

Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-S2P

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Axin GID 1-2 LP/pCS2MT

Plasmid

#16300

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 1-2 LP (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Expression

Mutation

GSK-3 interaction domain aa 320-429 with L to P m…

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

Plasmid

#13006

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 deltaCC (SULF2 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Cysteine 88 to Alanine

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27Linear-2, PolyU(0)

Plasmid

#166994

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(2)

Plasmid

#159480

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AIRAP 2: AIRAP(m1.9-T7)pGEX.KG

Plasmid

#21792

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge