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pDONR223_TERT_p.L593F

Plasmid

#82856

Purpose

Gateway Donor vector containing TERT, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, David Root, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TERT (TERT Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D516G, L593F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, gamma2 scFv [N452/81]

Plasmid

#182088

Purpose

Mammalian Expression of GABA(A)R, gamma2 scFv. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GABA(A)R, gamma2 (Homo sapiens) (GABRG2 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EEF2_SMARCA4

Plasmid

#205808

Purpose

Express mEGFP-tagged fusion protein, EEF2_SMARCA4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EEF2_SMARCA4 (EEF2, SMARCA4 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/30]

Plasmid

#206785

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/30 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HEPACAM2-COMP5AP-AviTag-9xHis

Plasmid

#157324

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HEPACAM2 (HEPACAM2 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HEPACAM2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156760

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HEPACAM2 (HEPACAM2 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/81]

Plasmid

#190556

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-CABC1

Plasmid

#23609

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CABC1 (COQ8A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pXPR_BRD051-sgMLH1-1

Plasmid

#125776

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-1 (MLH1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L [N452/81R]

Plasmid

#177554

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L/S [N452/73R]

Plasmid

#177553

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L/S (Human). Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L/S (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Δ754-756 dominant negative mutant

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L [N452/69]

Plasmid

#190331

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/69.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant Mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GABRG2 [N452/30]

Plasmid

#190330

Purpose

Mammalian Expression Plasmid of anti-GABRG2 (Human). Derived from hybridoma N452/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABRG2 (Homo sapiens) recombinant Mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

Plasmid

#183080

Purpose

rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

Depositor

Aviv Regev

Article

Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

Insert

TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)

Use

AAV; Homologous recombination donor template

Tags

Expression

Mutation

-124C>T promoter, Exon 1 C7C (C21>T21) sile…

Promoter

None for primary insert (Though partial TERT prom…

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEH_AAV_TERT_prom_C7C_1.8kb

Plasmid

#183079

Purpose

rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

Depositor

Aviv Regev

Article

Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

Insert

TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)

Use

AAV; Homologous recombination donor template

Tags

Expression

Mutation

Wildtype promoter, Exon 1 C7C (C21>T21) silent…

Promoter

None for primary insert (Though partial TERT prom…

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

Plasmid

#183081

Purpose

rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

Depositor

Aviv Regev

Article

Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

Insert

TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)

Use

AAV; Homologous recombination donor template

Tags

Expression

Mutation

-146C>T promoter, Exon 1 C7C (C21>T21) sile…

Promoter

None for primary insert (Though partial TERT prom…

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge