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Plasmid#74493PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNADepositorInsertCas9
UseTagst2a mCherryExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPRmTmG2
Plasmid#69992PurposeThe CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.DepositorInsertgRNA that targets near LoxP sites
UseTagsExpressionMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCUX1.1.0-gDNA
Plasmid#112434PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1DepositorInsertCUX1 (CUX1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAZ.1.0-gDNA
Plasmid#112455PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZDepositorInsertMAZ (MAZ Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHRB.1.0-gDNA
Plasmid#112429PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRBDepositorInsertTHRB (THRB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
B33
Plasmid#74008PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B51
Plasmid#74005PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B53
Plasmid#74006PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B31
Plasmid#74007PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBLO1811_arC9_noNLS_human
Plasmid#74494PurposeHuman codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNADepositorInsertarC9
UseCRISPRTagst2a mCherry (C-terminal on insert)ExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA1
Plasmid#78585Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBLO1811_Cas9_noNLS_human
Plasmid#74490PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNADepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_1
Plasmid#164986PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_class_I_2
Plasmid#164987PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genesDepositorInsertgRNA against HLA class I
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p330 VQR
Plasmid#101730PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorInsertSpCas9 VQR
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q, and T1337R mutations in Cas9)PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
C-12-BE
Plasmid#135040PurposeC-T base editing using LjCDA1L2_1 fused to C terminal of nCas9DepositorInsertLjCDA1L2_1
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-8-BE
Plasmid#135023PurposeC-T base editing using LpCDA1L1_1 fused to N terminal of nCas9DepositorInsertLpCDA1L1_1
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
C-1-BE
Plasmid#135029PurposeC-T base editing using PmCDA1L1_4 fused to C terminal of nCas9DepositorInsertPmCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-4-BE
Plasmid#135019PurposeC-T base editing using LjCDA1L1_4 fused to N terminal of nCas9DepositorInsertLjCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR_R4
Plasmid#124211PurposegRNA/Cas9 expression for 2nd editing using pSLHDR04DepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only