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Plasmid#77376Purpose3rd generation lentiviral gRNA plasmid targeting human STK31DepositorInsertSTK31 (STK31 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-E2F1-PIP3A
Plasmid#37142DepositorInsertE2F1-P!P3A (E2f1 Fly)
UseGatewayTagsExpressionMutationI153, Y156, Y157 to AlaninePromoterAvailable sinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
IV-GAA
Plasmid#43763DepositorInsertIV-GAA
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF1341
Plasmid#142376PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPOU5F1 (POU5F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_AB
Plasmid#148376PurposeInsect Expression of DmThor_50-83-YMVLtoAADDDepositorInsertDmThor_50-83-YMVLtoAADD (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA
Plasmid#194376PurposeSplit TEV assaysDepositorInsertGCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1-mCherry-Puro
Plasmid#193763PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsWTExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1ΔCy-mCherry-Puro
Plasmid#193764PurposeDoxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1ΔCy-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationCy motif (a.a. 68-70) mutated to alanine, a.a. 11…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389H
Plasmid#137658PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389HPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppil2.280.457.Y389W
Plasmid#137657PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppil2 (PPIL2 Human)
UseTags(His)6-thrombinExpressionBacterialMutation280-457-Y389WPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S2-Enz
Plasmid#183768PurposeExpresses SHIP2-Enzyme domain flanked by RBPDepositorInsertSHIP2 (INPPL1 Human)
UseTags6HIS, RBP (C-terminal) HRV3C cleavage site, and R…ExpressionBacterialMutationincludes aa K414 to aa V872 onlyPromoterT7lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_5071:Dendra
Plasmid#167376PurposeC-term fluorescent fusionDepositorInsertMSMEG_5071
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N130 miniABEMax V82G
Plasmid#135376PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 )DepositorInsertnSaCas9-ABEMax(V82G) N130
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-547-782
Plasmid#118376PurposeGateway compatible donor vector with KAT14-547-782 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 547-782)PromoterAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-96-3p
Plasmid#103760PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-96-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-96-3p target (MIR96 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-98-3p
Plasmid#103762PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-98-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-98-3p target (MIR98 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-98-5p
Plasmid#103763PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-98-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-98-5p target (MIR98 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-99b-3p
Plasmid#103766PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-99b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-99b-3p target (MIR99B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G
Plasmid#89376PurposeVector for reporter assay of one mouse super-enhancer regionDepositorInsertIrf2bpl (Irf2bpl Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
II-GCTC
Plasmid#43376DepositorInsertII-GCTC
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only