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Plasmid#96875PurposedHHR-bsgRNA- HEK4DepositorInsertdHHR-bsgRNA- HEK4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT055c
Plasmid#96876Purposetheophylline-agRNA-HEK3DepositorInserttheophylline-agRNA-HEK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057c
Plasmid#96870PurposeHHR-bsgRNA-FANCFDepositorInsertHHR-bsgRNA-FANCF
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057d
Plasmid#96871PurposedHHR-bsgRNA-FANCFDepositorInsertdHHR-bsgRNA-FANCF
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057a
Plasmid#96872PurposeHHR-bsgRNA-EMX1DepositorInsertHHR-bsgRNA-EMX1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057b
Plasmid#96873PurposedHHR-bsgRNA-EMX1DepositorInsertdHHR-bsgRNA-EMX1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057e
Plasmid#96868PurposeHHR-bsgRNA-HEK3DepositorInsertHHR-bsgRNA-HEK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT057f
Plasmid#96869PurposedHHR-bsgRNA-HEK3DepositorInsertdHHR-bsgRNA-HEK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT029d
Plasmid#96859Purpose(d)guanine _agRNA (GFP activation)DepositorInsert(d)guanine _agRNA (GFP activation)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT029c
Plasmid#96858Purposeguanine-agRNA (GFP activation)DepositorInsertguanine-agRNA (GFP activation)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT016g
Plasmid#96849Purposeprocessed HHR-sgRNA-GFPDepositorInsertprocessed HHR-sgRNA-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB8
Plasmid#82037PurposeHybrid CitA[1-193]-Tar[204-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB11
Plasmid#82040PurposeHybrid NarQ[1-217]-Tar[257-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKF293
Plasmid#72857Purposetemplate for N-terminal 3x FLAG tag, inducible Blasticidin resistanceDepositorInsert3xFlag tag
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKF294
Plasmid#72858Purposetemplate for N-terminal 1x Strep tag, inducible Blasticidin resistanceDepositorInsertsingle Strep tag
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTmaM4
Plasmid#67714PurposeExpression vector hexahistidine tagged variant of the Thermotoga maritima M-domain proteinDepositorInsertThermotoga marÃtima Ffh-M domain
UseTagshexahistidine tagExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET21a.AQ_1575
Plasmid#11416DepositorInserthypothetical (aq_1575 A. aeolicus)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pWUKI Cas1 V33A Cas2
Plasmid#80093PurposeEncodes Cas1 V33A and Cas2DepositorUseTagsExpressionBacterialMutationV33APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 V190I Cas2
Plasmid#80094PurposeEncodes Cas1 V190I and Cas2DepositorUseTagsExpressionBacterialMutationV190IPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 P202Q Cas2
Plasmid#80096PurposeEncodes Cas1 P202Q and Cas2DepositorUseTagsExpressionBacterialMutationP202QPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 P212L Cas2
Plasmid#80097PurposeEncodes Cas1 P212L and Cas2DepositorUseTagsExpressionBacterialMutationP212LPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 P282L Cas2
Plasmid#80098PurposeEncodes Cas1 P282L and Cas2DepositorUseTagsExpressionBacterialMutationP282LPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 P294H Cas2
Plasmid#80099PurposeEncodes Cas1 P294H and Cas2DepositorUseTagsExpressionBacterialMutationP294HPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 Cas2 T72I
Plasmid#80100PurposeEncodes Cas1 and Cas2 T72IDepositorUseTagsExpressionBacterialMutationT72IPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pWUKI Cas1 Cas2 G74A
Plasmid#80101PurposeEncodes Cas1 and Cas2 G74ADepositorUseTagsExpressionBacterialMutationG74APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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pIM23
Plasmid#132586PurposepZA4-oxySp-mCherry-TEVrs-LAADepositorInsertsoxySp-mCherry-TEVrs-LAA-TermT1
None
UseTagsExpressionBacterialMutationPromoternone and oxySpAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.wtTau
Plasmid#140424PurposeAAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTauDepositorInserteGFP-2A-Tau (MAPT Synthetic, Human)
UseAAVTagseGFPExpressionMutationPromoterCBAAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
OPEN HLA-A*02:01-BirA
Plasmid#215569PurposeE. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN HLA-A*02:01 BirA (HLA-A Human)
UseTagsBirAExpressionBacterialMutationGlycine 120 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB352_ZFcharm_Kv1_split-inteins
Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPaGE Pyl TAG FliC T248TAG
Plasmid#160089PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.DepositorInsertsPyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Threonine 248 to TAG stop-codon.PromoterPseudomonas aeruginosa Leu-tRNA native promoter a…Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaMKAR
Plasmid#205315PurposeFluorescent Reporter for CaMKII ActivityDepositorInsertCaMKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-Myc-SCAP
Plasmid#134285PurposeExpresses Myc-tagged SCAP in mammalian cellsDepositorUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTMB304_ZFcharm_Kv1_Prnp_SCR
Plasmid#220842PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB301_ZFcharm_Kv1_Prnp_SPM
Plasmid#220840PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-Flag-SREBP2
Plasmid#134284PurposeExpresses Flag-tagged SREBP2 in mammalian cellsDepositorInsertSREBP2 (Srebf2 Mouse)
UseTagsFlagExpressionMammalianMutationmouse SREBP2 precursorPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
Plasmid#154867PurposeFlp-dependent hM4D(Gi) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM4D(Gi)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_5SA-PolyA
Plasmid#112285PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)DepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with 5 Serines (61, 109, 127, 1…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
OPEN Beta2-microglobulin
Plasmid#215657PurposeE. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.DepositorInsertOPEN Beta2-microglobulin (B2M Human)
UseTagsExpressionBacterialMutationHistidine 31 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB400_ZFcharm_Prnp_DPM
Plasmid#220847PurposeAAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB399_ZFcharm_Prnp_SPM
Plasmid#220846PurposeAAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB340_ZFcharm_Kv1_Prnp_DPM
Plasmid#220843PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-Flag-SREBP1
Plasmid#134283PurposeExpresses Flag-tagged SREBP1 in mammalian cellsDepositorInsertSREBP1 (Srebf1 Mouse)
UseTagsFlagExpressionMammalianMutationmouse SREBP1 isoform a precursorPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL-C145A
Plasmid#160279PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC145APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL
Plasmid#160288PurposeAllows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-HCoV-NL63-3CL
Plasmid#160286PurposeAllows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-Bat-CoV-HKU9-3CL
Plasmid#160284PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only