-
Plasmid#21053DepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxVenus
Plasmid#103993PurposePlasmid encoding moxVenusDepositorInsertmoxVenus
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus ME
Plasmid#103989PurposePlasmid encoding mVenus MEDepositorInsertmVenus ME
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mClover3
Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUdioFRTeYFPW
Plasmid#133998Purposeexpresses eYFP upon coexpression with the recombinase flippase (Flp)DepositorInserteYFP
UseLentiviral; Flp/frtTagsExpressionMammalianMutationinverted and flanked by two incompatible FRT site…PromoterUbCAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1-sfGFP
Plasmid#103983PurposePlasmid encoding sfGFPDepositorInsertsfGFP
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express
Plasmid#103995PurposePlasmid encoding DsRed-ExpressDepositorInsertDsRed-Express
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-Katushka-9-5
Plasmid#104011PurposePlasmid encoding Katushka-9-5DepositorInsertKatushka9-5
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (K6S, N21D, R32G, L…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Clover
Plasmid#103991PurposePlasmid encoding CloverDepositorInsertClover
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Venus SX
Plasmid#103990PurposePlasmid encoding Venus SXDepositorInsertVenus SX
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-EGFP-6H
Plasmid#187001PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-mCherry-6H
Plasmid#187104PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVP65KR-gb-SacB(-)
Plasmid#171646PurposeEquipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.DepositorInsertMBP-mCherry-8xHis-SacB(-)
UseTags8xHisExpressionMutationinternal HindIII site in the SacB gene was remove…PromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS*11-yfp0
Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPLtetO1Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDC2.1.0-gDNA
Plasmid#113781PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2DepositorInsertCSDC2 (CSDC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GB4652
Plasmid#215266PurposeTU for the copper regulated expression of the flippase (FLP) recombinase.DepositorInsertCBS:miniDFR:FLP:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-VHL wt-pBabe-puro
Plasmid#19234DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwtPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…TagsExpressionBacterialMutationPromoterCOtetR-PIPL12Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCherry
Plasmid#99265PurposeContains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmCherry
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta1
Plasmid#16042DepositorInsertbeta1 (ITGB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP-T
Plasmid#103998PurposePlasmid encoding TagRFP-TDepositorInsertTagRFP-T
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorInsertGagZip-MCP
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
DR274-eGFP sgRNA
Plasmid#61051Purposefor generation of a eGFP specific sgRNA from a T7 promoterDepositorInsertEGFP sgRNA
UseCRISPRTagsExpressionMutationPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mKate2
Plasmid#104009PurposePlasmid encoding mKate2DepositorInsertmKate2
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, V45A, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorInsertmCAT1 (Slc7a1 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26b-SET
Plasmid#8822DepositorInsertSET (SET Human)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP
Plasmid#103997PurposePlasmid encoding TagRFPDepositorInsertTagRFP
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-cPla2-C2-EGFP-GH
Plasmid#187088PurposeProtein ExpressionDepositorInsertENSG00000116711 (PLA2G4A Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LR (bact: HyPer)
Plasmid#162069PurposeUsed for the diffuse visualization of H2O2 production in cells.DepositorInsertbeta actin 2 (actb2 Zebrafish)
UseGateway donor vectorTagsHyPerExpressionMutationPromoterT3Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean ME
Plasmid#103971PurposePlasmid encoding mCerulean MEDepositorInsertmCerulean ME
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB7 alpha5
Plasmid#16041DepositorInsertalpha5 (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express2
Plasmid#103999PurposePlasmid encoding DsRed-Express2DepositorInsertDsRed-Express2
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Flag-HA-Merlin
Plasmid#27104DepositorInsertMerlin (Nf2 Mouse)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5J
Plasmid#171949PurposeExpresses INPP5J in mammalian cellsDepositorInsertINPP5J (INPP5J Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Merlin-Flag-HA
Plasmid#27103DepositorInsertMerlin (Nf2 Mouse)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2
Plasmid#128166PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)DepositorInsertmTagBFP2-T2A-sfGFP-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAE teo-GFP
Plasmid#83958PurposeCan be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.DepositorInsertTetO-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorInsertipi1 (IPP2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only