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Plasmid#191599PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1333-DddA11-C
Plasmid#191600PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddA11-C
Plasmid#191601PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddA11-C
Plasmid#191602PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1333-DddA11-N
Plasmid#191607PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddA11-N
Plasmid#191608PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp32(L301G)
Plasmid#228209PurposeExpression in BL21(DE3) of single strand DNA binding protein L301GDepositorInsert61
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated leucine 301 to glycine (L301G), improves …PromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIB2
Plasmid#25451DepositorInsertPichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
UsePichia pastoris expressionTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIB4
Plasmid#25453DepositorInsertPichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
UsePichia pastoris expressionTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX
Plasmid#38042DepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIB3
Plasmid#25452DepositorInsertPichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
UsePichia pastoris expressionTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIB1
Plasmid#25450DepositorInsertPichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
UsePichia pastoris expressionTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutationPromoterAvailable sinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUlaR
Plasmid#216327PurposeT7 promoter driving expression of ulaRDepositorInsertL-Ascorbate-6-P-responsive transciptional repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad_UlaABC
Plasmid#216328PurposeArabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaCDepositorInsertUlaABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN859
Plasmid#190692PurposeMammalian expression of kinesin scFv(H2) fused with Fc domain of chicken IgYDepositorInsertkinesin scFv(H2)::Fc domain of chicken IgY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
NmCherry-SpVasa
Plasmid#66786Purposeexpression of mCherry in Sea Urchin germ cellsDepositorInsertVasa
UseSea urchin, zebrafish, xenopusTagsmCherryExpressionMammalianMutationPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-dtA
Plasmid#232236PurposeExpresses diphtheria toxin A subunit in Cre-positive cells.DepositorInsertDiphtheria toxin A subunit
UseAAVTagsExpressionMammalianMutationPromoterCAAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCVD434
Plasmid#63757PurposeEPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.DepositorInserteae probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-GCaMP6s
Plasmid#192945PurposeTo drive GCaMP6s under the control of mouse oxytocin promoterDepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB817 (pBra-Hfq)
Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Antp
Plasmid#86866PurposeBacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.DepositorInsertLinker-Antp
UseTagsAntpExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28b(+)/ADK C77S
Plasmid#201409PurposeOverexpresses Escherichia coli adenylate kinase C77SDepositorInsertEscherichia coli adenylate kinase C77S (adk Escherichia coli)
UseTagsExpressionBacterialMutationchanged cysteine 77 to serinePromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGFP-LacI
Plasmid#179510PurposeExpresses EGFP-LacI in mammalian cellsDepositorInsertLacI
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GLIS1
Plasmid#30166PurposeRetroviral expression of non-canonical human GLIS1DepositorInsertGLIS1 (GLIS1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBB75-ncRNA
Plasmid#194087PurposeExpresses Ec86 ncRNA in Escherichia coliDepositorInsertEc86 ncRNA
UseTagsExpressionMutationPromoterT7Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGLIS1
Plasmid#33273PurposeRetroviral expression of mouse Glis1DepositorInsertGLIS1, Gateway casette A (Glis1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only