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Showing: 41 - 60 of 128 results

  2. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid
    #128453
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128453-plasmid-map-sequence-id-261484

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  4. pCS2+-Zeo-miR-430-G-G mm

    Plasmid
    #221728
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-G-G mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    G-G mismatch of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221728-plasmid-map-sequence-id-449658

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  5. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Tags
    Expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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  7. pCdOpt-BMX

    Plasmid
    #203929
    Purpose
    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae
    Depositor
    Scott Briggs
    Article
    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11
    Insert
    BleMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    BleMX was codon optimized for Candida albicans or…
    Promoter
    Available since
    Dec. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    203929-plasmid-map-sequence-id-414816

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  8. pSLIK-Zeo

    Plasmid
    #25736
    Purpose
    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25736-plasmid-map-sequence-id-12061

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  9. pNAD1

    Plasmid
    #154347
    Purpose
    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance
    Depositor
    John van der Oost
    Article
    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.
    Insert
    ShBle
    Use
    Microalgae
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Violaxanthin Chlorophyll a Binding Protein Precur…
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154347-plasmid-map-sequence-id-316607

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  10. pEasyG2_zeo

    Plasmid
    #184913
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184913-plasmid-map-sequence-id-379508

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  11. pEasyG3_zeo

    Plasmid
    #184917
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184917-plasmid-map-sequence-id-368415

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  14. CDK1as_T2A_Zeo

    Plasmid
    #118596
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Depositor
    William Earnshaw
    Article
    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.
    Insert
    CDK1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    118596-plasmid-map-sequence-id-229650

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  15. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

    Plasmid
    #183607
    Purpose
    Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    LaG17 GFP nanobody SynNotch tTA
    Use
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183607-plasmid-map-sequence-id-361593

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  16. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

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Showing: 41 - 60 of 128 results