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Showing: 41 - 60 of 200 results

  4. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  13. pMOTag-M9-4H

    Plasmid
    #106379
    Purpose
    Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation
    Depositor
    Roberto Docampo
    Article
    Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:
    Insert
    GlmS with M9 mutation
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 2, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    106379-plasmid-map-sequence-id-204790

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  17. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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  18. pSY45

    Plasmid
    #48355
    Purpose
    Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48355-plasmid-map-sequence-id-70032

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  19. pDS66

    Plasmid
    #48364
    Purpose
    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48364-plasmid-map-sequence-id-70059

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  20. pDS67

    Plasmid
    #48365
    Purpose
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48365-plasmid-map-sequence-id-71676

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Showing: 41 - 60 of 200 results