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Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
UseTagsV5 His-tagExpressionBacterialMutationPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeE
Plasmid#129120PurposepBADHISTEV carrying pfeE for overexpression of PfeE in E. coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removedPromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam2_N_His8_eGFP_TEV
Plasmid#178806PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteinsDepositorTypeEmpty backboneUseTagsHis8, eGFP, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1
Plasmid#187456PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pegRNA2_tevopreQ1
Plasmid#212039PurposeBacterial expression of the pegRNA for genome editing in E. coliDepositorInsertpegRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSG
Plasmid#59731PurposeExpresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteriaDepositorInsertTJP1 (TJP1 Human)
UseTagsHIS and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam4_N_Flag_His8_eGFP_TEV
Plasmid#178808PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, His8, eGFP, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMGAHisTev5B
Plasmid#37221DepositorInserteIF5B
UseTagsHisExpressionMutationN-term truncation (contains aa 396-1002)PromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
PYL1_nTEVp
Plasmid#119213PurposeExpresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domainDepositorInsertsplit nTEVp in sufion with PYL1
UseTagsAU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABI_cTEVp
Plasmid#119214PurposeExpresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domainDepositorInsertsplit cTEVp in fusion with ABI
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_SCF2_LD6_CTEV
Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGTEVDNIb
Plasmid#122530PurposeBinary plasmid with a TEV (NIb deleted) cloneDepositorInsertTobacco etch virus (NIb deleted) cDNA
UsePotyvirus vectors to produce carotenoids in plantsTagsExpressionMutationPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP11_TEV
Plasmid#81243PurposeZipped GFP11 with TEV cleavage sequenceDepositorInsertzipped GFP11 with TEV cleavage sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ2
Plasmid#59734Purposeexpresses PDZ2 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 PDZ2 (TJP1 Human)
UseTagsHIS and TEVExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only